The Associated Press reported that the drone, from Tyndall Air Force Base in Panama City, Fla., crashed on the U.S. 98 highway in Florida's Panhandle.
The stretch of highway affected is an a rural part of Florida, but officials said the road could be closed for as long as 24 hours.
The drone has a 24-hour battery life and will become inactive after that, according to the AP.
The accident comes as the Federal Aviation Administration has been studying the potential impact of allowing non-military drones to fly beside commercial airplanes in U.S. airspace.
Supporters have said the use of drones in the commercial airspace would assist police departments in investigations. However, critics like the American Civil Liberties Union (ACLU) have raised privacy concerns.
The use of drones by the military became a hot topic in Congress earlier this year after the release of a memo from the Obama administration detailing potential strikes on American citizens overseas who are terrorism suspects.